|
Visit:
Pulmonary Pathology Online
Non-
Neoplastic Pulmonary Diseases: click here
HISTOCHEMICAL
STAIN:
Periodic acid schiff (PAS):
PAS-
Glycogen : Fungi
PAS with
diastase pretreatment
(Neutral
mucin): Pulmonary
Alveolar Proteinosis
Grocott :
For
Fungi ;
Pneumocystis
infection;
Ziel-Neelsen stain:
For mycobacterial organisms;
Tuberculosis
Elastic/van Gieson
stain for:
(collagen) - Fibrosis ; (elastin) -
Vasculature.
Eg. Interstitial fibrosis;
Organizing pneumonia; Vasculitis;
Pulmonary Hypertension ; chronic
lung rejection
Congo red
: for amyloid
(primary / secondary);
Perls’
prussian stain: for ferric iron. Eg.
Pulmonary Hemorrhage
;
ferruginous bodies.
von Kossa stain
phosphate;
IMMUNOHISTOCHEMICAL
/
fluorescence:
Cytomegalovirus
antibody :
Cytomegalovirus infection
OTHER ANALYSIS:
Polarising
microscopy for birefringent material:
Talc
; Mica ;
Pneumoconiosis
.
Mineral analysis:
(light or electron microscope)-
Diagnosis in
Asbestos-related
diseases.
Neoplastic Pulmonary Diseases: click here
HISTOCHEMICAL
STAIN:
Periodic acid
Schiff (PAS): Clear cell tumours
PAS with
diastase pretreatment
(Neutral
mucin): Adenocarcinoma
(Alcian blue pH
2.5 (Acid mucin):
Mesothelioma
(Mesothelioma-Online)
Combined Alcian blue/diastase
PAS.(basement membrane
/ neutral mucin): Adenoid
cystic Ca.
Argyrophil stains
such as Grimelius for neuroendocrine granules of carcinoid tumours.
Immunohistochemical
STAIN:
Immunohistochemical stains for
NSE and PGP 9.5 have limited value in distinguishing small cell
carcinoma from other types of carcinoma in small biopsies as they are
often non-specific and unreliable.
More specific markers, such as chromogranin and N-CAM, are not easily identified in poorly
differentiated tumours and small cell carcinoma.
CAM 5.2 and other cytokeratins give paranuclear dot positivity in small cell carcinoma
and can be used as part of a panel.
Immunostains can be usefully applied in
typing of neoplasms - CAM
5.2, leucocyte common antigen, desmin, synaptophysin,
chromogranin, N-CAM, S100, surfactant;
Lymphocyte
markers - B and T cell markers, light chains.
|
SUMMARY OF
IMMUNOSTAINS USEFUL IN THE DIAGNOSIS OF PULMONARY TUMOURS:
Histochemistry and Immunohistochemistry in the diagnosis of Mesothelioma:
click here
|
Carcinoembryonic antigen
Adenocarcinoma
Leu-M1
Ber-EP4
|
|
Surfactant
apoprotein A
Thyroid transcription Factor 1
(Thyroid and pulmonary origin)
|
|
Calretinin ;
Mesothelioma
Cytokeratin 5 / 6 ;
Thrombomodulin ;
|
|
Chromogranin A ; Synaptophysin ; CD56 :
Neuroendocrine Tumours. |
|
|
